u6 promoter:grna expression cassettes Search Results


u6 promoter:grna expression cassettes  (GenScript corporation)
90
GenScript corporation u6 promoter:grna expression cassettes
U6 Promoter:Grna Expression Cassettes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/u6 promoter:grna expression cassettes/product/GenScript corporation
Average 90 stars, based on 1 article reviews
u6 promoter:grna expression cassettes - by Bioz Stars, 2026-03
90/100 stars
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pgcas9 -expressing cassette and u6 promoter: grna backbone  (GenScript corporation)
90
GenScript corporation pgcas9 -expressing cassette and u6 promoter: grna backbone
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Pgcas9 Expressing Cassette And U6 Promoter: Grna Backbone, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgcas9 -expressing cassette and u6 promoter: grna backbone/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pgcas9 -expressing cassette and u6 promoter: grna backbone - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
grna expression cassette promoter u6.6 medicago truncatula  (Medicago)
90
Medicago grna expression cassette promoter u6.6 medicago truncatula
The T-DNA structure <t>of</t> <t>PgCas9</t> / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic <t>tRNA-gRNA</t> (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.
Grna Expression Cassette Promoter U6.6 Medicago Truncatula, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/grna expression cassette promoter u6.6 medicago truncatula/product/Medicago
Average 90 stars, based on 1 article reviews
grna expression cassette promoter u6.6 medicago truncatula - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


The T-DNA structure of PgCas9 / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic tRNA-gRNA (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.

Journal: Frontiers in Plant Science

Article Title: Efficient Multi-Sites Genome Editing and Plant Regeneration via Somatic Embryogenesis in Picea glauca

doi: 10.3389/fpls.2021.751891

Figure Lengend Snippet: The T-DNA structure of PgCas9 / PaU6 . The hygromycin phosphate transferase ( hpt ) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled 35S promoter and terminated by the Nos terminator. The PaU6 promoter was used to drive the expression of polycistronic tRNA-gRNA (PTG), and two Bsa I sites were designed between the PaU6 promoter and the gRNA backbone for assembling the PTG into the binary vector. Eco RI, Sac I, Nco I, Bam HI, Sal I, and Hind III were restriction enzymes. The left and right borders of T-DNA were designated as LB and RB, respectively.

Article Snippet: The final sequence containing PgCas9 -expressing cassette and U6 promoter: gRNA backbone was synthesized by Genscript Biotechnology Co., Ltd (Nanjing, China) and ligated into the modified pCAMBIA1300 vector, in which the Bsa I site was removed with QuickMutation TM (D0206, Beyotime Biotechnology, Shanghai, China), following the manufacturer's introduction.

Techniques: Marker, Expressing, Plasmid Preparation